Berättelser från Casumos universum - Casumobloggen

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CB15" , Caulobacter sp. Caulobacter crescentus is a single-celled, small bacterium that divides asymmetrically producing cells that differ in structure and function - it is an ideal model system for the mechanisms of asymmetric cell division and has been studied thoroughly.

The genome of Caulobacter crescentus is structured in a circular chromosome and is comprised of 4,, bp encoding for 3, genes.

It was found that the C. Caulobacter also uses DNA methylation as a regulatory mechanism in cell cycle progression. Caulobacter also has several genes and clusters of genes that are essential for survival in habitats with poor nutrients.

Some of these genes encode for proteins involved in chemotaxis, outer membrane channel function, degradation of compounds with aromatic rings, and the breakdown of plant-derived carbon sources list from Nierman et al.

Others are involved in extracytoplasmic function sigma factors, which allow the bacterium to respond to "a wide range of environmental fluctuations" Nierman et al.

Caulobacter are Gram-negative, rod-like cells that can be flagellated in a polar manner or have a stalk. Caulobacter lack intracellular organelles.

They are hereotrophic aerobes and can be found in aquatic environments attached to particulate matter, plant materials, or other microorganisms by its stalk.

Caulobacter asymmetrically divides to produce two types of daughter cells that are functionally and structurally different. The cylindrical body of a stalk cell is approximately 0.

The swarmer cell is smaller than the stalk cell initially; the different hydrodynamics due to the different morphologies between the swarmer and stalk cells allow swarmers to be isolated relatively easily Stanford.

Caulobacter generally live in a dilute aquatic environment where the most common limiting nutrient is phosphorus, an essential element for healthy growth.

Lack of this nutrient induce Caulobacter to dramatically elongate its stalk up to 30 times longer than those in phosphate-rich medium Brun et al.

Caulobacter asymmetrically divides to produce a motile swarmer cell and a stalk cell. The swarmer cell, which has a flagellum, swims for about minutes before shedding the flagellum and differentiating into a stalk cell.

The flagellum is ejected from the swamer by the destruction of the structures MS ring at the base of the flagellum. Within the swarmer cell, chromosomes do not replicate; however, chromosome replication begins immediately in the daughter cell with the stalk and when the swarmer loses its tail Stanford.

The stalk adheres to surfaces through an adhesive organelle called the holdfast. Several two-component signal transduction proteins are involved in the cell cycle progression by accumulating at one or both poles "in a spatial and temporal pattern that is reproduced during each cycle" Jacobs-Wagner In addition to this, DNA methylation is main component of signaling differentiation.

Throughout the cell cycle, the chromosome progressively goes from being fully methylated to hemimethylated during DNA replication - this results in differential binding of regulatory proteins to activate or repress transcription.

Berättelser Från Casumos Universum - Casumobloggen Video

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